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CDC45L Recombinant Antibody, PerCP-Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-61334r-percp-cy7
Product Name CDC45L Recombinant Antibody, PerCP-Cy7 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation PerCP-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CDC45
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8318
Swiss Prot O75419
Subcellular location Cytoplasm, Nucleus
Synonyms CDC45L; CDC45L2; PORC-PI-1; CDC45.
Background Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is CDC45L Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-61334R-PerCP-Cy7) validated for, and at what dilution?

bsm-61334R-PerCP-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDC45L Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-61334R-PerCP-Cy7) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-61334R-PerCP-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDC45L Recombinant Antibody, PerCP-Cy7 Conjugated (bsm-61334R-PerCP-Cy7)?

KLH conjugated synthetic peptide derived from human CDC45

What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bsm-61334R-PerCP-Cy7)?

PerCP-Cy7 on bsm-61334R-PerCP-Cy7: excitation / emission 482nm/780nm.