CDC45L Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61334r-rbitc |
| Product Name | CDC45L Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDC45 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8318 |
| Swiss Prot | O75419 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CDC45L; CDC45L2; PORC-PI-1; CDC45. |
| Background | Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CDC45L Recombinant Antibody, RBITC Conjugated (bsm-61334R-RBITC) validated for, and at what dilution?
bsm-61334R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDC45L Recombinant Antibody, RBITC Conjugated (bsm-61334R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-61334R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDC45L Recombinant Antibody, RBITC Conjugated (bsm-61334R-RBITC)?
KLH conjugated synthetic peptide derived from human CDC45
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61334R-RBITC)?
RBITC on bsm-61334R-RBITC: excitation / emission 570nm/595nm.