CDC27 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61336r-cy5-100ul |
| Product Name | CDC27 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDC27 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 996 |
| Swiss Prot | P30260 |
| Subcellular location | Nucleus |
| Synonyms | CDC27Hs; Cell division cycle protein 27 homolog; HNUC; APC3; ANAPC3. |
| Background | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CDC27 Recombinant Antibody, Cy5 Conjugated (bsm-61336R-Cy5) validated for, and at what dilution?
bsm-61336R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDC27 Recombinant Antibody, Cy5 Conjugated (bsm-61336R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61336R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDC27 Recombinant Antibody, Cy5 Conjugated (bsm-61336R-Cy5)?
KLH conjugated synthetic peptide derived from human CDC27
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61336R-Cy5)?
Cy5 on bsm-61336R-Cy5: excitation / emission 625,650nm/670nm.