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Caspase 2 Recombinant Antibody, PerCP Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61341r-percp
Product Name Caspase 2 Recombinant Antibody, PerCP Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Caspase 2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 835
Swiss Prot P42575
Subcellular location Cytoplasm, Nucleus
Synonyms Caspase-2; Caspase 2; Caspase2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; CASP2; ICH1; NEDD2.
Background Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is Caspase 2 Recombinant Antibody, PerCP Conjugated (bsm-61341R-PerCP) validated for, and at what dilution?

bsm-61341R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Caspase 2 Recombinant Antibody, PerCP Conjugated (bsm-61341R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61341R-PerCP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Caspase 2 Recombinant Antibody, PerCP Conjugated (bsm-61341R-PerCP)?

KLH conjugated synthetic peptide derived from human Caspase 2

What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61341R-PerCP)?

PerCP on bsm-61341R-PerCP: excitation / emission 482nm/677nm.