CDCA5 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61346r-cy3 |
| Product Name | CDCA5 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sororin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 113130 |
| Swiss Prot | Q96FF9 |
| Subcellular location | Nucleus |
| Synonyms | CDCA5; Cell division cycle associated protein 5; Cell division cycle-associated protein 5; p35; Sororin. |
| Background | Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CDCA5 Recombinant Antibody, Cy3 Conjugated (bsm-61346R-Cy3) validated for, and at what dilution?
bsm-61346R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDCA5 Recombinant Antibody, Cy3 Conjugated (bsm-61346R-Cy3) react with?
Tested reactivity: Human.
How should bsm-61346R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDCA5 Recombinant Antibody, Cy3 Conjugated (bsm-61346R-Cy3)?
KLH conjugated synthetic peptide derived from human Sororin
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61346R-Cy3)?
Cy3 on bsm-61346R-Cy3: excitation / emission 512,550nm/570,615nm.