CDC34 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61348r-cy3 |
| Product Name | CDC34 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDC34 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 997 |
| Swiss Prot | P49427 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CDC34; Cell division cycle 34; UBC3; UBCH3; Ubiquitin-protein ligase R1; UBE2R1; E2-CDC34; Ubiquitin carrier protein. |
| Background | Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is CDC34 Recombinant Antibody, Cy3 Conjugated (bsm-61348R-Cy3) validated for, and at what dilution?
bsm-61348R-Cy3 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDC34 Recombinant Antibody, Cy3 Conjugated (bsm-61348R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61348R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDC34 Recombinant Antibody, Cy3 Conjugated (bsm-61348R-Cy3)?
KLH conjugated synthetic peptide derived from human CDC34
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61348R-Cy3)?
Cy3 on bsm-61348R-Cy3: excitation / emission 512,550nm/570,615nm.