Phospho-CDC42 (Ser71) Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61349r-bf555 |
| Product Name | Phospho-CDC42 (Ser71) Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cdc42 around the phosphorylation site of S71 |
| Modification Site | Ser71 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 998 |
| Swiss Prot | P60953,P63000 |
| Subcellular location | Cytoplasm |
| Synonyms | CDC42; CDC42Hs; G25K; TKS; MIG5; Ras like protein TC25. |
| Background | Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-CDC42 (Ser71) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61349R-BF555) validated for, and at what dilution?
bsm-61349R-BF555 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CDC42 (Ser71) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61349R-BF555) react with?
Tested reactivity: Human.
How should bsm-61349R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CDC42 (Ser71) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61349R-BF555)?
KLH conjugated synthetic peptide derived from human Cdc42 around the phosphorylation site of S71
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61349R-BF555)?
AbBy Fluor-555 on bsm-61349R-BF555: excitation / emission 553nm/568nm.