Phospho-CBL (Tyr774) Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61354r-100uL |
| Product Name | Phospho-CBL (Tyr774) Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CBL around the phosphorylation site of Y774 |
| Modification Site | Tyr774 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified Polyclonal A |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 867 |
| Swiss Prot | P22681 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Casitas B lineage lymphoma proto oncogene; CBL2; E3 ubiquitin protein ligase CBL; Oncogene CBL2; Proto oncogene c CBL; RING finger protein 55; RNF55; Signal transduction protein CBL. |
| Background | Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is Phospho-CBL (Tyr774) Recombinant Antibody (bsm-61354R) validated for, and at what dilution?
bsm-61354R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CBL (Tyr774) Recombinant Antibody (bsm-61354R) react with?
Tested reactivity: Human.
How should bsm-61354R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Phospho-CBL (Tyr774) Recombinant Antibody (bsm-61354R)?
KLH conjugated synthetic peptide derived from human CBL around the phosphorylation site of Y774