Phospho-CBL (Ser669) Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61355r-bf555 |
| Product Name | Phospho-CBL (Ser669) Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CBL around the phosphorylation site of S669 |
| Modification Site | Ser669 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 867 |
| Swiss Prot | P22681 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Casitas B lineage lymphoma proto oncogene; cbl; CBL2; E3 ubiquitin protein ligase CBL; Oncogene CBL2; Proto oncogene c CBL; RING finger protein 55; RNF55; Signal transduction protein CBL. |
| Background | Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is Phospho-CBL (Ser669) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61355R-BF555) validated for, and at what dilution?
bsm-61355R-BF555 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CBL (Ser669) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61355R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61355R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CBL (Ser669) Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61355R-BF555)?
KLH conjugated synthetic peptide derived from human CBL around the phosphorylation site of S669
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61355R-BF555)?
AbBy Fluor-555 on bsm-61355R-BF555: excitation / emission 553nm/568nm.