Caspase 14 Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61356r-percp |
| Product Name | Caspase 14 Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Caspase 14 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23581 |
| Swiss Prot | P31944 |
| Subcellular location | Cytoplasm |
| Synonyms | CASP14; Caspase-14; Caspase 14; MICE; CASP-14. |
| Background | Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum.Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin.In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Caspase 14 Recombinant Antibody, PerCP Conjugated (bsm-61356R-PerCP) validated for, and at what dilution?
bsm-61356R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase 14 Recombinant Antibody, PerCP Conjugated (bsm-61356R-PerCP) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-61356R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase 14 Recombinant Antibody, PerCP Conjugated (bsm-61356R-PerCP)?
KLH conjugated synthetic peptide derived from human Caspase 14
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61356R-PerCP)?
PerCP on bsm-61356R-PerCP: excitation / emission 482nm/677nm.