MGMT Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61358r-cy3 |
| Product Name | MGMT Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MGMT |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4255 |
| Swiss Prot | P16455 |
| Subcellular location | Nucleus |
| Synonyms | MGMT |
| Background | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is MGMT Recombinant Antibody, Cy3 Conjugated (bsm-61358R-Cy3) validated for, and at what dilution?
bsm-61358R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MGMT Recombinant Antibody, Cy3 Conjugated (bsm-61358R-Cy3) react with?
Tested reactivity: Human.
How should bsm-61358R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MGMT Recombinant Antibody, Cy3 Conjugated (bsm-61358R-Cy3)?
KLH conjugated synthetic peptide derived from human MGMT
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61358R-Cy3)?
Cy3 on bsm-61358R-Cy3: excitation / emission 512,550nm/570,615nm.