PCGF4/BMI1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61372r-cy3 |
| Product Name | PCGF4/BMI1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BMI1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 100532731 |
| Swiss Prot | P35226 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | BMI1 ; Polycomb complex protein BMI 1; RING finger protein 51; RNF51; PCGF4. |
| Background | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is PCGF4/BMI1 Recombinant Antibody, Cy3 Conjugated (bsm-61372R-Cy3) validated for, and at what dilution?
bsm-61372R-Cy3 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PCGF4/BMI1 Recombinant Antibody, Cy3 Conjugated (bsm-61372R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-61372R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PCGF4/BMI1 Recombinant Antibody, Cy3 Conjugated (bsm-61372R-Cy3)?
KLH conjugated synthetic peptide derived from human BMI1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61372R-Cy3)?
Cy3 on bsm-61372R-Cy3: excitation / emission 512,550nm/570,615nm.