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CD247 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated

Applications

  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-61374r-apc-cy5
Product Name CD247 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
FCM IF(ICC)
Reactivity Human
Specifications
Conjugation APC-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD247
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 919
Swiss Prot P20963
Subcellular location Cell membrane
Synonyms CD3-zeta; T-cell receptor T3 zeta chain; T3Z; TCRZ; CD247.
Background Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID). A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
Application Dilution
FCM 1:20-100
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CD247 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61374R-APC-Cy5) validated for, and at what dilution?

bsm-61374R-APC-Cy5 is validated for FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD247 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61374R-APC-Cy5) react with?

Tested reactivity: Human.

How should bsm-61374R-APC-Cy5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD247 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61374R-APC-Cy5)?

KLH conjugated synthetic peptide derived from human CD247

What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61374R-APC-Cy5)?

APC-Cy5 on bsm-61374R-APC-Cy5: excitation / emission 650nm/670nm.