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CD247 Recombinant Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-61374r-bf647
Product Name CD247 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD247
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 919
Swiss Prot P20963
Subcellular location Cell membrane
Synonyms CD3-zeta; T-cell receptor T3 zeta chain; T3Z; TCRZ; CD247.
Background Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID). A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is CD247 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61374R-BF647) validated for, and at what dilution?

bsm-61374R-BF647 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD247 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61374R-BF647) react with?

Tested reactivity: Human.

How should bsm-61374R-BF647 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD247 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61374R-BF647)?

KLH conjugated synthetic peptide derived from human CD247

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61374R-BF647)?

AbBy Fluor-647 on bsm-61374R-BF647: excitation / emission 650nm/665nm.