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LAMP1 Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-61375r-cy3
Product Name LAMP1 Recombinant Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LAMP1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3916
Swiss Prot P11279
Synonyms LAMP-1; CD107 antigen-like family member A; CD107a; LAMP1.
Background Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is LAMP1 Recombinant Antibody, Cy3 Conjugated (bsm-61375R-Cy3) validated for, and at what dilution?

bsm-61375R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LAMP1 Recombinant Antibody, Cy3 Conjugated (bsm-61375R-Cy3) react with?

Tested reactivity: Human.

How should bsm-61375R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LAMP1 Recombinant Antibody, Cy3 Conjugated (bsm-61375R-Cy3)?

KLH conjugated synthetic peptide derived from human LAMP1

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61375R-Cy3)?

Cy3 on bsm-61375R-Cy3: excitation / emission 512,550nm/570,615nm.