SP1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61380r-rbitc |
| Product Name | SP1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SP1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6667 |
| Swiss Prot | P08047 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | TSFP1; Transcription factor SP1; transcription factor Sp1. |
| Background | Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SP1 Recombinant Antibody, RBITC Conjugated (bsm-61380R-RBITC) validated for, and at what dilution?
bsm-61380R-RBITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SP1 Recombinant Antibody, RBITC Conjugated (bsm-61380R-RBITC) react with?
Tested reactivity: Human.
How should bsm-61380R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SP1 Recombinant Antibody, RBITC Conjugated (bsm-61380R-RBITC)?
KLH conjugated synthetic peptide derived from human SP1
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61380R-RBITC)?
RBITC on bsm-61380R-RBITC: excitation / emission 570nm/595nm.