NEDD8 Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61385r-bf555 |
| Product Name | NEDD8 Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NEDD8 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4738 |
| Swiss Prot | Q15843 |
| Subcellular location | Nucleus |
| Synonyms | NEDD8; Nedd-8. |
| Background | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53.Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is NEDD8 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61385R-BF555) validated for, and at what dilution?
bsm-61385R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NEDD8 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61385R-BF555) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61385R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEDD8 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61385R-BF555)?
KLH conjugated synthetic peptide derived from human NEDD8
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61385R-BF555)?
AbBy Fluor-555 on bsm-61385R-BF555: excitation / emission 553nm/568nm.