Dnmt1 Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61399r-rbitc |
| Product Name | Dnmt1 Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DNMT1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1786 |
| Swiss Prot | P26358 |
| Subcellular location | Nucleus |
| Synonyms | ADCADN; CXXC finger protein 9; CXXC9; DNA methyltransferase 1; DNA MTase; Dnmt1o; HSN1E; M.HsaI; MCMT; Met1; MommeD2. |
| Background | Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Dnmt1 Recombinant Antibody, RBITC Conjugated (bsm-61399R-RBITC) validated for, and at what dilution?
bsm-61399R-RBITC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Dnmt1 Recombinant Antibody, RBITC Conjugated (bsm-61399R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61399R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Dnmt1 Recombinant Antibody, RBITC Conjugated (bsm-61399R-RBITC)?
KLH conjugated synthetic peptide derived from human DNMT1
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61399R-RBITC)?
RBITC on bsm-61399R-RBITC: excitation / emission 570nm/595nm.