SMC3 Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61431r-apc-100uL |
| Product Name | SMC3 Recombinant Antibody, APC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMC3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9126 |
| Swiss Prot | Q9UQE7 |
| Subcellular location | Nucleus |
| Synonyms | SMC protein 3; Bamacan; SMC3; BAM; BMH; CSPG6; SMC3L1. |
| Background | Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SMC3 Recombinant Antibody, APC Conjugated (bsm-61431R-APC) validated for, and at what dilution?
bsm-61431R-APC is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMC3 Recombinant Antibody, APC Conjugated (bsm-61431R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61431R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMC3 Recombinant Antibody, APC Conjugated (bsm-61431R-APC)?
KLH conjugated synthetic peptide derived from human SMC3
What are the excitation and emission wavelengths of the APC conjugate (bsm-61431R-APC)?
APC on bsm-61431R-APC: excitation / emission 650nm/660nm.