Peroxiredoxin-6 Recombinant Antibody

$4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Peroxiredoxin-6) monoclonal Antibody, unconjugated (bsm-61449R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Peroxiredoxin-6) monoclonal Antibody, unconjugated (bsm-61449R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Peroxiredoxin-6 antibody (bsm-61449R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Peroxiredoxin-6 antibody (bsm-61449R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

$25 ug total protein per lane of various lysates (see on figure) probed with Peroxiredoxin-6 monoclonal antibody, unconjugated (bsm-61449R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Peroxiredoxin-6 monoclonal antibody, unconjugated (bsm-61449R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61449r-TR
Product Name	Peroxiredoxin-6 Recombinant Antibody
Applications	WB, FCM, IF(ICC), IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PRDX6
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9588
Swiss Prot	P30041
Subcellular location	Cytoplasm
Synonyms	Peroxiredoxin-6; Antioxidant protein 2; Liver 2D page spot 40; NSGPx; PRDX6; AOP2; KIAA.
Background	Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IF(ICC)	IFICC1:50-200
IP	IP1:20-50