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Hepcidin-25 Recombinant Antibody, APC-Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Rat
Overview
Catalog # bsm-61456r-apc-Cy5.5
Product Name Hepcidin-25 Recombinant Antibody, APC-Cy5.5 Conjugated
Applications
WB
Reactivity Human, Rat
Specifications
Conjugation APC-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hepcidin
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 57817
Swiss Prot P81172
Subcellular location Secreted
Synonyms Hepcidin; Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor; PLTR; Hepcidin-25; Hepc25; Hepcidin-20; Hepc20; HAMP; HEPC; LEAP1.
Background Hepcidin, also known as HAMP, HEPC, LEAP1 or HFE2B, is an 84 amino acid secreted protein that regulates iron-related signaling events. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Hepcidin-25 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-61456R-APC-CY5-5) validated for, and at what dilution?

BSM-61456R-APC-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Hepcidin-25 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-61456R-APC-CY5-5) react with?

Tested reactivity: Human, Rat.

How should BSM-61456R-APC-CY5-5 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Hepcidin-25 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-61456R-APC-CY5-5)?

KLH conjugated synthetic peptide derived from human Hepcidin

What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BSM-61456R-APC-CY5-5)?

APC-Cy5.5 on BSM-61456R-APC-CY5-5: excitation / emission 650nm/694nm.