RAC1/2/3 Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61458r-pe-Cy5.5 |
| Product Name | RAC1/2/3 Recombinant Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Rac1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5879 |
| Swiss Prot | P63000,P15153,P60763 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Cell migration-inducing gene 5 protein; EN-7; GX; p21-Rac1; p21-Rac2; p21-Rac3; RAC1; RAC2; RAC3; TC25. |
| Background | In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is RAC1/2/3 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61458R-PE-CY5-5) validated for, and at what dilution?
BSM-61458R-PE-CY5-5 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAC1/2/3 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61458R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-61458R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAC1/2/3 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61458R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human Rac1
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-61458R-PE-CY5-5)?
PE-Cy5.5 on BSM-61458R-PE-CY5-5: excitation / emission 488nm/694nm.