Ku70 / XRCC6 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61460r-bf488-100uL |
| Product Name | Ku70 / XRCC6 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Ku70 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2547 |
| Swiss Prot | P12956 |
| Subcellular location | Nucleus |
| Synonyms | CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen 70kDa; Ku autoantigen, 70kDa; Ku p70; Ku70; Kup70; TLAA; XRCC6. |
| Background | Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Ku70 / XRCC6 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61460R-BF488) validated for, and at what dilution?
bsm-61460R-BF488 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ku70 / XRCC6 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61460R-BF488) react with?
Tested reactivity: Human.
How should bsm-61460R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ku70 / XRCC6 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61460R-BF488)?
KLH conjugated synthetic peptide derived from human Ku70
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61460R-BF488)?
AbBy Fluor-488 on bsm-61460R-BF488: excitation / emission 499nm/519nm.