RPE65 Recombinant Antibody

$Western blot analysis of mouse eyeball lysate. Using RPE65 (bsm-61470R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of mouse eyeball lysate. Using RPE65 (bsm-61470R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

$25 ug total protein per lane of various lysates (see on figure) probed with RPE65 monoclonal antibody, unconjugated (bsm-61470R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with RPE65 monoclonal antibody, unconjugated (bsm-61470R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPE65 Monoclonal Antibody, Unconjugated (bsm-61470R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPE65 Monoclonal Antibody, Unconjugated (bsm-61470R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bsm-61470r
Product Name	RPE65 Recombinant Antibody
Applications	WB IP
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RPE65
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6121
Swiss Prot	Q16518
Subcellular location	Cytoplasm, Cell membrane
Synonyms	LCA2; mRPE65; p63; rd12; Retinal pigment epithelium specific 61 kDa protein; RP20; RPE65; sRPE65.
Background	Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore
Application Dilution
WB	1:300-5000
IP	1-2ug