RPE65 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61470r-cy3 |
| Product Name | RPE65 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RPE65 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6121 |
| Swiss Prot | Q16518 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | LCA2; mRPE65; p63; rd12; Retinal pigment epithelium specific 61 kDa protein; RP20; RPE65; sRPE65. |
| Background | Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is RPE65 Recombinant Antibody, Cy3 Conjugated (bsm-61470R-Cy3) validated for, and at what dilution?
bsm-61470R-Cy3 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RPE65 Recombinant Antibody, Cy3 Conjugated (bsm-61470R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bsm-61470R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RPE65 Recombinant Antibody, Cy3 Conjugated (bsm-61470R-Cy3)?
KLH conjugated synthetic peptide derived from human RPE65
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61470R-Cy3)?
Cy3 on bsm-61470R-Cy3: excitation / emission 512,550nm/570,615nm.