p53BP1 Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61471r-bf350-100uL |
| Product Name | p53BP1 Recombinant Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TP53BP1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7158 |
| Swiss Prot | Q12888 |
| Subcellular location | Nucleus |
| Synonyms | Tumor suppressor p53-binding protein 1; 53 BP1; p53-binding protein 1; p53BP1; TP53BP1; p53-BP1; p202. |
| Background | Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is p53BP1 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61471R-BF350) validated for, and at what dilution?
bsm-61471R-BF350 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p53BP1 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61471R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61471R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p53BP1 Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61471R-BF350)?
KLH conjugated synthetic peptide derived from human TP53BP1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-61471R-BF350)?
AbBy Fluor-350 on bsm-61471R-BF350: excitation / emission 346nm/442nm.