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PRKAR2B Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61473r-cy3-100uL
Product Name PRKAR2B Recombinant Antibody, Cy3 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC) IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PRKAR2B
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5577
Swiss Prot P31323
Synonyms AI451071; cAMP dependent protein kinase type II beta regulatory chain; Pkarb2; PRKAR2B; Protein kinase cAMP dependent regulatory type II beta; RATDNA; RII beta.
Background Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200
IF

Frequently asked questions

Which applications is PRKAR2B Recombinant Antibody, Cy3 Conjugated (bsm-61473R-Cy3) validated for, and at what dilution?

bsm-61473R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PRKAR2B Recombinant Antibody, Cy3 Conjugated (bsm-61473R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61473R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PRKAR2B Recombinant Antibody, Cy3 Conjugated (bsm-61473R-Cy3)?

KLH conjugated synthetic peptide derived from human PRKAR2B

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61473R-Cy3)?

Cy3 on bsm-61473R-Cy3: excitation / emission 512,550nm/570,615nm.