ATG3 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61474r-cy5-100uL |
| Product Name | ATG3 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATG3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64422 |
| Swiss Prot | Q9NT62 |
| Subcellular location | Cytoplasm |
| Synonyms | APG3 autophagy 3 like; APG3 like; APG3, S. cerevisiae, homolog of; APG3-like; APG3L; Apg3p; ATG3 autophagy related 3 homolog. |
| Background | E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ATG3 Recombinant Antibody, Cy5 Conjugated (bsm-61474R-Cy5) validated for, and at what dilution?
bsm-61474R-Cy5 is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG3 Recombinant Antibody, Cy5 Conjugated (bsm-61474R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61474R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATG3 Recombinant Antibody, Cy5 Conjugated (bsm-61474R-Cy5)?
KLH conjugated synthetic peptide derived from human ATG3
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61474R-Cy5)?
Cy5 on bsm-61474R-Cy5: excitation / emission 625,650nm/670nm.