Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

ATG10 Recombinant Antibody

Western blot analysis of HeLa cell lysate. Using ATG10 (bsm-61475R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Western blot analysis of HeLa cell lysate. Using ATG10 (bsm-61475R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
The K562 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ATG10 antibody (bsm-61475R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The K562 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ATG10 antibody (bsm-61475R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-61475r
Product Name ATG10 Recombinant Antibody
Applications
WB IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ATG10
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 83734
Swiss Prot Q9H0Y0
Subcellular location Cytoplasm
Synonyms APG10L;ATG10 autophagy related 10 homolog (S. cerevisiae); ATG10 autophagy related 10 homolog; Autophagy related protein 10.
Background E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3.
Application Dilution
WB 1:300-5000
IP 1-2ug

Frequently asked questions

Which applications is ATG10 Recombinant Antibody (bsm-61475R) validated for, and at what dilution?

bsm-61475R is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ATG10 Recombinant Antibody (bsm-61475R) react with?

Tested reactivity: Human.

How should bsm-61475R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise ATG10 Recombinant Antibody (bsm-61475R)?

KLH conjugated synthetic peptide derived from human ATG10

Search our catalog of hundreds of thousands of products