Cyclin H Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61481r-pe-Cy5.5 |
| Product Name | Cyclin H Recombinant Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cyclin H |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 902 |
| Swiss Prot | P51946 |
| Subcellular location | Nucleus |
| Synonyms | CAK; CAK complex subunit; CDK activating kinase; CDK activating kinase complex subunit; CyclinH; MO15 associated protein; p34; p36; p37. |
| Background | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is Cyclin H Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61481R-PE-CY5-5) validated for, and at what dilution?
BSM-61481R-PE-CY5-5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cyclin H Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61481R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-61481R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cyclin H Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61481R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human Cyclin H
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-61481R-PE-CY5-5)?
PE-Cy5.5 on BSM-61481R-PE-CY5-5: excitation / emission 488nm/694nm.