Clathrin heavy chain Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61487r-cy5-100uL |
| Product Name | Clathrin heavy chain Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Clathrin heavy chain 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1213 |
| Swiss Prot | Q00610,P53675 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CHC17; Clathrin heavy chain; Clathrin heavy chain like 1; CLH17; CLH22; CLTC; CLTCL; CLTD. |
| Background | Clathrin heavy chain proteins are composed of several functional domains, including a carboxy-terminal region that permits interaction with other heavy chain proteins within a triskelion, and a globular amino-terminal region that associates with other vesicle proteins. Adaptor proteins, such as AP2, epsin and EPS15, are responsible for the recruitment of vesicle proteins to sites of pit formation and the assembly of the clathrin-coated vesicle. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Clathrin heavy chain Recombinant Antibody, Cy5 Conjugated (bsm-61487R-Cy5) validated for, and at what dilution?
bsm-61487R-Cy5 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Clathrin heavy chain Recombinant Antibody, Cy5 Conjugated (bsm-61487R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61487R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Clathrin heavy chain Recombinant Antibody, Cy5 Conjugated (bsm-61487R-Cy5)?
KLH conjugated synthetic peptide derived from human Clathrin heavy chain 1
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61487R-Cy5)?
Cy5 on bsm-61487R-Cy5: excitation / emission 625,650nm/670nm.