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CHD4 Recombinant Antibody

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-61493r-TR
Product Name CHD4 Recombinant Antibody
Applications
WB FCM IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHD4
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1108
Swiss Prot Q14839
Subcellular location Cytoplasm, Nucleus
Synonyms ATP dependent helicase CHD4; CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta.
Background ATP-dependent helicase that binds and distorts nucleosomal DNA.Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666.Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is CHD4 Recombinant Antibody (bsm-61493R) validated for, and at what dilution?

bsm-61493R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHD4 Recombinant Antibody (bsm-61493R) react with?

Tested reactivity: Human.

How should bsm-61493R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise CHD4 Recombinant Antibody (bsm-61493R)?

KLH conjugated synthetic peptide derived from human CHD4