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BubR1 Recombinant Antibody, APC Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61498r-apc
Product Name BubR1 Recombinant Antibody, APC Conjugated
Applications
WB IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BUB1B
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 701
Swiss Prot O60566
Subcellular location Cytoplasm, Nucleus
Synonyms hBUBR1; Mitotic checkpoint kinase MAD3L Protein SSK1; BUB1B; BUBR1; MAD3L; SSK1.
Background Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle.
Application Dilution
WB 1:300-5000
IF
Flow-Cyt 1ug/Test

Frequently asked questions

Which applications is BubR1 Recombinant Antibody, APC Conjugated (bsm-61498R-APC) validated for, and at what dilution?

bsm-61498R-APC is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BubR1 Recombinant Antibody, APC Conjugated (bsm-61498R-APC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61498R-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BubR1 Recombinant Antibody, APC Conjugated (bsm-61498R-APC)?

KLH conjugated synthetic peptide derived from human BUB1B

What are the excitation and emission wavelengths of the APC conjugate (bsm-61498R-APC)?

APC on bsm-61498R-APC: excitation / emission 650nm/660nm.