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CAPN1 Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-61500r-bf405-100uL
Product Name CAPN1 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Calpain 1 catalytic subunit
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 823
Swiss Prot P07384
Subcellular location Cytoplasm, Cell membrane
Synonyms Calpain-1 catalytic subunit; CANP; CANP small subunit; CANPL1; CAPN1; Micromolar Calpain; Mu Calpain; muCANP.
Background Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).
Application Dilution
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CAPN1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61500R-BF405) validated for, and at what dilution?

bsm-61500R-BF405 is validated for IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CAPN1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61500R-BF405) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-61500R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CAPN1 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61500R-BF405)?

KLH conjugated synthetic peptide derived from human Calpain 1 catalytic subunit

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-61500R-BF405)?

AbBy Fluor-405 on bsm-61500R-BF405: excitation / emission 401nm/421nm.