CAPN1 Recombinant Antibody

$4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CAPN1) monoclonal Antibody, unconjugated (bsm-61500R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CAPN1) monoclonal Antibody, unconjugated (bsm-61500R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-61500r
Product Name	CAPN1 Recombinant Antibody
Applications	ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Calpain 1 catalytic subunit
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	823
Swiss Prot	P07384
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Calpain-1 catalytic subunit; CANP; CANP small subunit; CANPL1; CAPN1; Micromolar Calpain; Mu Calpain; muCANP.
Background	Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).
Application Dilution
ICC/IF	1:50-100