B7-H3/CD276 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61503r-cy7 |
| Product Name | B7-H3/CD276 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD276 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80381 |
| Swiss Prot | Q5ZPR3 |
| Subcellular location | Cell membrane |
| Synonyms | 4Ig B7 H3; B7 homolog 3; B7H3; B7RP-2; CD276 antigen; CD276 molecule. |
| Background | May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is B7-H3/CD276 Recombinant Antibody, Cy7 Conjugated (bsm-61503R-Cy7) validated for, and at what dilution?
bsm-61503R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does B7-H3/CD276 Recombinant Antibody, Cy7 Conjugated (bsm-61503R-Cy7) react with?
Tested reactivity: Human.
How should bsm-61503R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise B7-H3/CD276 Recombinant Antibody, Cy7 Conjugated (bsm-61503R-Cy7)?
KLH conjugated synthetic peptide derived from human CD276
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61503R-Cy7)?
Cy7 on bsm-61503R-Cy7: excitation / emission 743nm/767nm.