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alpha Sarcoglycan Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bsm-61505r-cy3
Product Name alpha Sarcoglycan Recombinant Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P)
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human alpha Sarcoglycan
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6442
Swiss Prot Q16586
Subcellular location Cytoplasm, Cell membrane
Synonyms 50DAG; 50kD DAG; adhalin; ADL; Alpha SG; Alpha-sarcoglycan; Asg; DAG2; DMDA2; Dystroglycan 2; LGMD2D; SCARMD1; Sgca.
Background Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is alpha Sarcoglycan Recombinant Antibody, Cy3 Conjugated (bsm-61505R-Cy3) validated for, and at what dilution?

bsm-61505R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does alpha Sarcoglycan Recombinant Antibody, Cy3 Conjugated (bsm-61505R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bsm-61505R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise alpha Sarcoglycan Recombinant Antibody, Cy3 Conjugated (bsm-61505R-Cy3)?

KLH conjugated synthetic peptide derived from human alpha Sarcoglycan

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61505R-Cy3)?

Cy3 on bsm-61505R-Cy3: excitation / emission 512,550nm/570,615nm.