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BNIP1 Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61513r-cy3
Product Name BNIP1 Recombinant Antibody, Cy3 Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BNIP1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 662
Swiss Prot Q12981
Subcellular location Cytoplasm
Synonyms BCL2 adenovirus E1B 19kD interacting protein 1; BNIP1; Nip1; sec20; SEC20L; Transformation-related gene 8 protein; TRG8; Vesicle transport protein SEC20.
Background SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is BNIP1 Recombinant Antibody, Cy3 Conjugated (bsm-61513R-Cy3) validated for, and at what dilution?

bsm-61513R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BNIP1 Recombinant Antibody, Cy3 Conjugated (bsm-61513R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61513R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BNIP1 Recombinant Antibody, Cy3 Conjugated (bsm-61513R-Cy3)?

KLH conjugated synthetic peptide derived from human BNIP1

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61513R-Cy3)?

Cy3 on bsm-61513R-Cy3: excitation / emission 512,550nm/570,615nm.