APG4B Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with APG4B monoclonal antibody, unconjugated (bsm-61514R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with APG4B monoclonal antibody, unconjugated (bsm-61514R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61514r-TR
Product Name	APG4B Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG4B
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23192
Swiss Prot	Q9Y4P1
Subcellular location	Cytoplasm
Synonyms	Cysteine protease ATG4B; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B.
Background	Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy.
Application Dilution
WB	1:500-2000