PDK1 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61525r-bf647 |
| Product Name | PDK1 Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDK1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5163 |
| Swiss Prot | Q15118 |
| Subcellular location | Cytoplasm |
| Synonyms | Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDH kinase 1; Pdk1; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1. |
| Background | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is PDK1 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61525R-BF647) validated for, and at what dilution?
bsm-61525R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDK1 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61525R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61525R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDK1 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61525R-BF647)?
KLH conjugated synthetic peptide derived from human PDK1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61525R-BF647)?
AbBy Fluor-647 on bsm-61525R-BF647: excitation / emission 650nm/665nm.