ITK Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61531r-bf555 |
| Product Name | ITK Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ITK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3702 |
| Swiss Prot | Q08881 |
| Subcellular location | Nucleus |
| Synonyms | EMT; Homolog of mouse T cell itk/tsk; IL 2 inducible T cell kinase; Itk; Kinase EMT; LPFS1; LYK; PSCTK2; T cell specific kinase; TSK; Tyrosine protein kinase ITK/TSK. |
| Background | Interleukin-2 inducible T-cell kinase (Itk, Emt or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain and a SH3 and SH2 domain followed by a carboxy-terminal kinase domain. Tec, Rlk and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is ITK Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61531R-BF555) validated for, and at what dilution?
bsm-61531R-BF555 is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ITK Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61531R-BF555) react with?
Tested reactivity: Human.
How should bsm-61531R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ITK Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61531R-BF555)?
KLH conjugated synthetic peptide derived from human ITK
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61531R-BF555)?
AbBy Fluor-555 on bsm-61531R-BF555: excitation / emission 553nm/568nm.