PDCD4 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61534r-bf594-100uL |
| Product Name | PDCD4 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
IF
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDCD4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27250 |
| Swiss Prot | Q53EL6 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Death up-regulated gene protein; Dug; H731; Ma3; Neoplastic transformation inhibitor; Nuclear antigen H731; Pdcd4; Programmed cell death protein 4; Tis. |
| Background | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
| IF | |
Frequently asked questions
Which applications is PDCD4 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61534R-BF594) validated for, and at what dilution?
bsm-61534R-BF594 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDCD4 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61534R-BF594) react with?
Tested reactivity: Human, Mouse.
How should bsm-61534R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDCD4 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61534R-BF594)?
KLH conjugated synthetic peptide derived from human PDCD4
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-61534R-BF594)?
AbBy Fluor-594 on bsm-61534R-BF594: excitation / emission 590nm/617nm.