CEACAM6 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with CEACAM6 monoclonal antibody, unconjugated (bsm-61545R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CEACAM6 monoclonal antibody, unconjugated (bsm-61545R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human lung;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human thyroid tumor;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human thyroid tumor;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CEACAM6 Monoclonal Antibody, Unconjugated (bsm-61545R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IP
IF
ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-61545r-TR
Product Name	CEACAM6 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CEACAM6
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4680
Swiss Prot	P40199
Subcellular location	Cell membrane
Synonyms	CD66c; CEACAM6; CEAL; NCA; Non specific cross reacting antigen; Normal cross reacting antigen.
Background	Cell surface glycoprotein that plays a role in cell adhesion and tumor progression.Intercellular adhesion occurs in a calcium- and fibronectin-independent manner.Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF
ICC/IF	1:50-100