DHFR Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61547r-apc-cy5-100uL |
| Product Name | DHFR Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Dihydrofolate reductase |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1719 |
| Swiss Prot | P00374 |
| Subcellular location | Cytoplasm |
| Synonyms | DHFR; DHFRP1; Dihydrofolate reductase; DYR. |
| Background | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DHFR Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61547R-APC-Cy5) validated for, and at what dilution?
bsm-61547R-APC-Cy5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DHFR Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61547R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61547R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DHFR Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61547R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human Dihydrofolate reductase
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61547R-APC-Cy5)?
APC-Cy5 on bsm-61547R-APC-Cy5: excitation / emission 650nm/670nm.