CYP3A4 Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61566r-percp-100uL |
| Product Name | CYP3A4 Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cytochrome P450 3A4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1576 |
| Swiss Prot | P08684 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase. |
| Background | A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is CYP3A4 Recombinant Antibody, PerCP Conjugated (bsm-61566R-PerCP) validated for, and at what dilution?
bsm-61566R-PerCP is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP3A4 Recombinant Antibody, PerCP Conjugated (bsm-61566R-PerCP) react with?
Tested reactivity: Human.
How should bsm-61566R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP3A4 Recombinant Antibody, PerCP Conjugated (bsm-61566R-PerCP)?
KLH conjugated synthetic peptide derived from human Cytochrome P450 3A4
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61566R-PerCP)?
PerCP on bsm-61566R-PerCP: excitation / emission 482nm/677nm.