ROCK2 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61567r-cy5-100uL |
| Product Name | ROCK2 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ROCK2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9475 |
| Swiss Prot | O75116 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | p164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha. |
| Background | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ROCK2 Recombinant Antibody, Cy5 Conjugated (bsm-61567R-Cy5) validated for, and at what dilution?
bsm-61567R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ROCK2 Recombinant Antibody, Cy5 Conjugated (bsm-61567R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61567R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ROCK2 Recombinant Antibody, Cy5 Conjugated (bsm-61567R-Cy5)?
KLH conjugated synthetic peptide derived from human ROCK2
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61567R-Cy5)?
Cy5 on bsm-61567R-Cy5: excitation / emission 625,650nm/670nm.