Emerin Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Emerin Monoclonal Antibody, Unconjugated(bsm-61601R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Emerin Monoclonal Antibody, Unconjugated(bsm-61601R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with Emerin monoclonal antibody, unconjugated (bsm-61601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Emerin monoclonal antibody, unconjugated (bsm-61601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-61601r-100ul
Product Name	Emerin Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Emerin
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2010
Swiss Prot	P50402
Subcellular location	Nucleus, Cell membrane
Synonyms	EDMD; Emd; Emerin; Emery Dreifuss muscular dystrophy; STA.
Background	Emerin may regulate gene expression through binding to other transcriptional regulators. Emerin binds to ŚÂ-catenin and inhibits its nuclear accumulation. Recent studies demonstrate that Emerin is required for HIV-1 infectivity.
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500
IF(ICC)	IFICC1:50-200