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SLIT2 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with SLIT2 monoclonal antibody, unconjugated (bsm-61608R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with SLIT2 monoclonal antibody, unconjugated (bsm-61608R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SLIT2 Monoclonal Antibody, Unconjugated (bsm-61608R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IP

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61608r-100uL
Product Name SLIT2 Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P) IP
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Slit 2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 9353
Swiss Prot O94813
Subcellular location Secreted
Synonyms Drad 1; Slil3; Slit homolog 2 (Drosophila); Slit homolog 2 protein C-product; Slit2 .
Background Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:200-800
IHC-F IHC-F1:200-800
IF(IHC-P) IFIHC-P1:200-800
IP IP1:20-50

Frequently asked questions

Which applications is SLIT2 Recombinant Antibody (bsm-61608R) validated for, and at what dilution?

bsm-61608R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:200-800); IHC-F(IHC-F) (1:200-800); IF(IHC-P)(IF(IHC-P)) (1:200-800); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SLIT2 Recombinant Antibody (bsm-61608R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61608R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise SLIT2 Recombinant Antibody (bsm-61608R)?

KLH conjugated synthetic peptide derived from human Slit 2

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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