Phospho-CRK (Tyr221) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61622r-apc-cy5-100uL |
| Product Name | Phospho-CRK (Tyr221) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CRK around the phosphorylation site of Y221 |
| Modification Site | Tyr221 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1398 |
| Swiss Prot | P46108 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; CRK; CRKII; Proto-oncogene c-Crk. |
| Background | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-CRK (Tyr221) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61622R-APC-Cy5) validated for, and at what dilution?
bsm-61622R-APC-Cy5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CRK (Tyr221) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61622R-APC-Cy5) react with?
Tested reactivity: Human.
How should bsm-61622R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CRK (Tyr221) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61622R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human CRK around the phosphorylation site of Y221
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61622R-APC-Cy5)?
APC-Cy5 on bsm-61622R-APC-Cy5: excitation / emission 650nm/670nm.