CRK Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61631r-percp-100uL |
| Product Name | CRK Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CRK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1398 |
| Swiss Prot | P46108 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CRK; CRKII; p38. |
| Background | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CRK Recombinant Antibody, PerCP Conjugated (bsm-61631R-PerCP) validated for, and at what dilution?
bsm-61631R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CRK Recombinant Antibody, PerCP Conjugated (bsm-61631R-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61631R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CRK Recombinant Antibody, PerCP Conjugated (bsm-61631R-PerCP)?
KLH conjugated synthetic peptide derived from human CRK
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61631R-PerCP)?
PerCP on bsm-61631R-PerCP: excitation / emission 482nm/677nm.